Checkpoint Signalling: Mad2 Conformers and Signal Propagation
نویسنده
چکیده
The spindle checkpoint protein Mad2 has a tendency to form multimers and adopts at least two structural conformations. New work highlights the importance of the Mad2-Mad2 interaction, and suggests how spindle checkpoint signals are propagated away from kinetochores.
منابع مشابه
The Mad1/Mad2 Complex as a Template for Mad2 Activation in the Spindle Assembly Checkpoint
BACKGROUND The spindle assembly checkpoint (SAC) imparts fidelity to chromosome segregation by delaying anaphase until all sister chromatid pairs have become bipolarly attached. Mad2 is a component of the SAC effector complex that sequesters Cdc20 to halt anaphase. In prometaphase, Mad2 is recruited to kinetochores with the help of Mad1, and it is activated to bind Cdc20. These events are linke...
متن کاملAccumulation of Mad2–Cdc20 complex during spindle checkpoint activation requires binding of open and closed conformers of Mad2 in Saccharomyces cerevisiae
The spindle assembly checkpoint (SAC) coordinates mitotic progression with sister chromatid alignment. In mitosis, the checkpoint machinery accumulates at kinetochores, which are scaffolds devoted to microtubule capture. The checkpoint protein Mad2 (mitotic arrest deficient 2) adopts two conformations: open (O-Mad2) and closed (C-Mad2). C-Mad2 forms when Mad2 binds its checkpoint target Cdc20 o...
متن کاملThe Mad2 Conformational Dimer: Structure and Implications for the Spindle Assembly Checkpoint
The 25 kDa Mad2 protein is a key player in the spindle assembly checkpoint, a safeguard against chromosome segregation errors in mitosis. Mad2 combines three unusual properties. First, Mad2 adopts two conformations with distinct topologies, open (O) and closed (C) Mad2. Second, C-Mad2 forms topological links with its two best-characterized protein ligands, Mad1 and Cdc20. Third, O-Mad2 and C-Ma...
متن کاملp31 Blocks Mad2 Activation through Structural Mimicry
The status of spindle checkpoint signaling depends on the balance of two opposing dynamic processes that regulate the highly unusual two-state behavior of Mad2. In mitosis, a Mad1-Mad2 core complex recruits cytosolic Mad2 to kinetochores through Mad2 dimerization and converts Mad2 to a conformer amenable to Cdc20 binding, thereby facilitating checkpoint activation. p31 inactivates the checkpoin...
متن کاملp31comet Blocks Mad2 Activation through Structural Mimicry
The status of spindle checkpoint signaling depends on the balance of two opposing dynamic processes that regulate the highly unusual two-state behavior of Mad2. In mitosis, a Mad1-Mad2 core complex recruits cytosolic Mad2 to kinetochores through Mad2 dimerization and converts Mad2 to a conformer amenable to Cdc20 binding, thereby facilitating checkpoint activation. p31(comet) inactivates the ch...
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ورودعنوان ژورنال:
- Current Biology
دوره 15 شماره
صفحات -
تاریخ انتشار 2005